HiSense™ Gas Analysis
Unlike conventional exhaust gas analysis, the HiSense™ analysis systems from HiTec Zang also offer the biological variables OTR, CTR and RQ. The devices are suited for R&D and laboratory applications and for production and quality assurance.
Measuring equipment
Sensors are used for oxygen analysis which are distinguished by their low interference, low-noise readings and high long-term stability.
NDIR photometers with a minimum of zero and increase errors are used for the CO2 measurement. Through a new form of special measuring method even the precise measurement of very low activity both for small differences and for small measured gas flows are possible.
Advantages
- 2 to 5 measuring channels for up to 4 fermenters with a common gas feed or 2 fermenters with separate gas feeds
- High-resolution measurement (HiSense™-c)
- Humidity correction / gas drying (HiSense™-c)
- "Genuine" OUR, CER and RQ measurement (HiSense™-c)
- Low interference
- Overpressure possible
- Wear-free sensors
- Compact
- Additional functions can be integrated
- Optionally freely programmable
- Many possibilities for couplings
- Data export
Downloads
In the cell culture version the correction of unavoidable influences such as overall pressure, humidity etc. with a high degree of accuracy is enabled thanks to additional measuring equipment and offsetting. The influences may be very large in conventional exhaust gas analysis, low metabolic rates and long cultivation periods and may make a comparison of cultivations more difficult or even impossible, meaning that the use of exhaust gas analysis for quality assurance becomes impossible. Not until the influence variables have been corrected is it possible to gain meaningful readings.
The HiSense™-c precision gas analysis system for cell culture and fermentation applications additionally provides an online determination of the true CO2 production (CER) in the carbonate buffer systems normally found in a cell culture (optional pH input required). In addition, the errors are corrected which make valid measurements impossible as a consequence of the shifting of the HCO3 /CO2 balance by metabolic products (e. g. lactate, NH3 ). Only in this way is a determination of the actual RQ possible.
The high measuring resolution enables meaningful measurements under normal conditions, even for the low breathing rates of animal cell cultures and in a very broad range (reactor volumes of 1 l up to more than 100,000 l), depending on the model.
The software carries out all calculations online including all corrections. All readings are saved in a database and may be progressively displayed on the x-t plotter.
An export as a file (csv, txt and other formats) is possible.
General
| Type | HiSense™-m for microbiology | HiSense™-c for cell culture and microbiology |
|---|---|---|
| Dimensions (D x W x H) | 380x 450 x 150 mm | 380x 450 x 280 mm |
| Power supply | 110/230 V, 50/60 Hz | 110/230 V, 50/60 Hz |
| Power consumption | about 3 A | about 3 A |
| Temperature range | 0...30 °C | 0...30 °C |
| Degree of protection | IP20 | IP20 |
Measuring Channels
| Gas feed/exhaust gas | 2 to 5 (optional) channels for measuring in gas feed and/or exhaust gas | 2...4 (optional) channels for measurement in the gas feed and exhaust gas of 1...4 fermenters or several gas feed flows |
|---|---|---|
| Working range of flow | 18...210 ln/h (l/h at 0 °C / 1,013 mbar) | Min. of 1.8 ln/h (l/h at 0 °C / 1,013 mbar) |
| Pressure loss | <1,000 Pa | |
| Operating pressure | Atmospherically up to 3 bar absolute | Atmospherically up to 3 bar absolute |
O2 Measurement
| Principle | Solid electrolyte system | Solid electrolyte system, optionally paramagnetic |
|---|---|---|
| Measuring range | 0...75 Vol.% | 0...75 vol.%, paramagnetic 0...100 Vol.% |
| Measurement uncertainty | ± 1 % of measuring range | ± 1 % of measuring range |
| Resolution | 0.2 vol.% for 20 % O2 , better on request | 0.2 vol.% for 20 % O2, better on request |
| Long-term drift | <0.2 Vol. % | < 0.2 Vol. % |
| Relevant resolution | Difference, humidity and pressure corrected, resolution approx. 0.02 % | Difference, humidity and pressure corrected, resolution approx. 0.01 % |
Mass Flow Meter
| Principle | Thermal mass flow measurement, CO2 corrected | Thermal mass flow measurement, CO2 corrected | ||
|---|---|---|---|---|
| Standard | All channels | All channels | ||
| Measurement uncertainty | Better than 2 % of range | Better than 2 % of range | ||
| Reproducibility | 0.2 % of range | 0.2 % of range | ||
OTR-CTR
| Measurement interval | 1...10 minutes depending on the hardware and software equipment | approx. 10 min, for 2 fermenters approx. 15 min |
|---|---|---|
| Sensitivity of OTR** | About 1 x 10-3 mol/l/h at 1 l culture volume, 5vvh (V gas/V liquid/h) gas injection rate | About 1 x 10-5 mol/l/h at 1 l culture volume, 5vvh (V gas / V liquid / h) gas injection rate |
| Sensitivity of CTR** | About 1 x 10-3 mol/l/h at 1 l culture volume, 5vvh gas injection rate | About 1 x 10-5 mol/l/h at 1 l culture volume, 5vvh gas injection rate |
| Warm-up period | 60 min | 120 min |
| Measured variable | pH value, OTR, CTR, OT, CT, RQ, growth rate and many secondary variables such as dissolved O2 , CO2 ... | pH value, OTR, CTR, OT, CT, TQ, CER, CE, RQ (corrected TQ), growth rate and many secondary variables such as dissolved O2 , CO... |
|---|
CO2 Measurement
| Principle | NDIR | NDIR |
|---|---|---|
| Measuring range | 0...18 Vol%, others on request | 0...18 Vol%, others on request |
| Measurement uncertainty | ± 1 % of measuring range | ± 0.5 % of measuring range |
| Resolution | 0.02 vol.% for 5 % CO2 | 0.01 vol.% for 5 % CO2 |
| Principle | <± 0.2 % | <± 0.05 % |
| Automatic compensation | Humidity, absolute pressure | Humidity, absolute pressure |
| Relevant resolution | Difference, humidity and pressure corrected, resolution approx. 0.02 % | Difference, humidity and pressure corrected, resolution approx. 0.01 % |
Operation/Control
| PC | Operation using supplied software with graphical user interface Data export to Microsoft® Excel® or as a text file | Operation using supplied software with graphical user interface Data export to Microsoft® Excel® or as a text file |
|---|
| Product code | Description |
|---|---|
| IL-HISENSE-m-2 | Gas analysis system for the determination of O2 and CO2, OTR, CTR and RQ, 2-channel design |
| IL-HISENSE-m-3 | Gas analysis system for the determination of O2 and CO2, OTR, CTR and RQ, 3-channel design |
| IL-HISENSE-m-4 | Gas analysis system for the determination of O2 and CO2, OTR, CTR and RQ, 4-channel design |
| IL-HISENSE-m-5 | Gas analysis system for the determination of O2 and CO2, OTR, CTR and RQ, 5-channel design |
| IL-HISENSE-c-2 | Gas analysis system for the determination of O2 and CO2, OTR, CTR and RQ, 2-channel design |
| IL-HISENSE-c-4 | Gas analysis system for the determination of O2 and CO2, OTR, CTR and RQ, 4-channel design |
| IL-HISENSE-c-8 | Gas analysis system for the determination of O2 and CO2, OTR, CTR and RQ, 8-channel design |
Gmix™ Gas Mixture Stations
The Gmix™ precision gas mixture station was especially developed for applications which demand an extremely high degree of accuracy and reproducibility of the gas mixture.
The gas mixture station may be supplied in various configurations. The gas mixture of up to four gases can be individually adjusted for each output.
All gas inputs may be allocated as desired with the gases air, nitrogen, oxygen and carbon dioxide and are calibrated for these gases at the factory. Up to 10 gases can be calibrated per input. Other gases are possible on request. The units can be preselected to mln/min, ln/min and ln/h.
The Gmix™ gas mixture station is available both as an OmniFerm® and RAMOS® extension and as an independent system.
Advantages
- Best possible accuracy and reproducibility
- Especially even dosing
- Compact construction
- Automatic monitoring and protective function
- Self-explanatory user interface
Downloads
Application
The areas of application for a precision gas mixture station include all forms of biological fermentation in which individual and highly accurate gas mixtures must be used. The gas mixture station is able to be additionally used in physical equipment and trace gas analysis due to its highly precise gas mixture. A further use is the calibration of gas sensors.
High-quality measurements are only possible to be achieved for exhaust gas analysis in fermenter systems with the use of a precision gas mixture station.
Advantages
Flows and percentages are displayed and set in a self-explanatory dialogue form. The concentrations are maintained at a constant level by high-quality mass flow meters (no multiplex operation!). As a result particularly even and accurate dosing is guaranteed. Only very little space is required to be taken up thanks to the compact design.
Monitoring and Protection Devices
If the set outlet pressure is exceeded, the gas feed is automatically cut off and an alarm signal triggered. After 30 seconds und when the pressure has fallen below the limit value, the gas feed is re-opened.
The chosen set points are graphically displayed. The measured flows are shown numerically and the chronological sequence will be displayed graphically.
On request the devices may be fitted with further options such as
- An adjustment of the system to special gases
- Up to 10 gas data sets for each input
- Systems with several outputs by a cascade of several devices or as a special construction
- Analogue inputs 4...20 mA
- Regulator for dissolved O2 and pH
- Digital input for external control stand-by, ramp start and stop
General
| Dimensions (D x W x H) | 320 x 240 x 150 mm up to 4 inputs (on request: 5 to 6 inputs, width approx. 340 mm) |
|---|---|
| Weight | approx. 6 kg, up to 4 inputs, 1 output |
| Power supply | 110/230 V, 50/60 Hz |
| Power consumption | 0.35 A |
| Temperature range | 0 °C to 40 °C |
| Degree of protection | IP20 |
Monitoring
| Output pressure | Limiting value for alarm signalling and switching off adjustable, accuracy approx. 100 mbar relative |
|---|---|
| Flow | Actual value display with alarm signalling when exceeded and fallen below |
Materials
| Materials in contact with medium | Standard: Aluminium, brass (partially nickel-plated), FKM (Viton), stainless steel, PUR, Si, Si3N4, SiOx, epoxy Option: Metals + PUR, replaceable by stainless steel |
|---|
Inputs
| Quantity | 2 to 4 gases, pneumatic fittings 6/4 (other connections on request) |
|---|---|
| Gases | Air, nitrogen (N2), oxygen(O2), carbon dioxide (CO2) as standard, non-condensing, additional gases on request. Optional (multigas) up to 10 gases can be calibrated for each input |
| Input pressure | According to specification, pressure resistent up to 11 bar absolute (from approx. 40 slm* to 9 bar absolute) |
Outputs
| Quantity | 1 (optionally up to 4), pneumatic fitting 6/4 (other connections on request) |
|---|---|
| Output pressure | According to specification up to 10 bar absolute (from approx. 40 slm* to 8.5 bar absolute) |
| Min. pressure difference | approx. 0.5...1 bar, according to specifications |
| Control principle | Thermal mass flow controller, each component is individually regulated at each output, no multiplex operation |
| Pre-filter | Input filter 20μm |
Flows
| Work area | Standard: Final value 0.05 slm*...5 slm*, on request up to 200 slm* and larger Dynamics 1:50; option: smaller and larger, Dynamics 1:100 |
|---|---|
| Uncertainty | Standard <1.0 % of final value, optional ± 0.3 % of final value + 0.5 % of reading |
| Reproducibility | <0.1 % of final value |
| Long term stability | <1 % of reading |
| Accuracy of settings | Flow according to range, components 0.01 % |
| Rise time | <1 s for ± 2 % of set point |
| Warm-up period | 10 s |
| Temperature coefficient | 0.025 %/°C |
| Pressure coefficient | -0.2 %/bar |
Operation
| PC | Operation using supplied software with graphical user interface Data export to Microsoft® Excel® |
|---|---|
| Interfaces | Optional: Remote control via a serial COM interface or a Windows named pipe, via a command set in accordance with NAMUR remote control possible using other programs |
| Product code | Description |
|---|---|
| IL-GMIX21 | Standard gas mixture station (2 inputs, 1 output) for non-condensing/corrosive gases(Air, O2, N2, CO2), 1 gas/input, inputs 0.05 to 5 slm final value*, uncertainty ± 1.0 % of final value, dynamics 1:50 |
| IL-GMIX31 | Standard gas mixture station (3 inputs, 1 output) for non-condensing/corrosive gases (Air, O2, N2, CO2), 1 gas/input, inputs 0.05 to 5 slm final value*, uncertainty ± 1.0 % of final value, dynamics 1:50 |
| IL-GMIX41 | Standard gas mixture station (4 inputs, 1 output) for non-condensing/corrosive gases (Air, O2, N2, CO2), 1 gas/input, inputs 0.05 to 5 slm final value*, uncertainty ± 1.0 % of final value, dynamics 1:50 |
| IL-GMIX21-X | Gas mixture station with increased accuracy (2 inputs, 1 output) for non-condensing/corrosive gases (Air, O2, N2, CO2), 1 gas/input, inputs 0.05 to 5 slm final value*, uncertainty ± (0.3 % of final value + 0.5 % of measured value), dynamics 1:100 |
| IL-GMIX31-X | Gas mixture station with increased accuracy (3 inputs, 1 output) for non-condensing/corrosive gases (Air, O2, N2, CO2), 1 gas/input, inputs 0.05 to 5 slm final value*, uncertainty ± (0.3 % of final value + 0.5 % of measured value), dynamics 1:100 |
| IL-GMIX41-X | Gas mixture station with increased accuracy (4 inputs, 1 output) for non-condensing/corrosive gases (Air, O2, N2, CO2), 1 gas/input, inputs 0.05 to 5 slm final value*, uncertainty ± (0.3 % of final value + 0.5 % of measured value), dynamics 1:100 |
| IL-GMIX-SA | Gmix™‐Option stand-alone system with touch-screen operating unit |