OmniFerm® Mini Parallel Fermenter Systems
The OmniFerm® mini parallel fermenter system is distinguished by the particularly small footprint, enabling several bioreactors to fit onto one laboratory workbench. This mini-culture system closes the gap between shake flasks and a laboratory fermenter. Knowledge gained in shake cultures may now be validated in the next-largest scale with little need for material and with great time savings.
The modular construction and the numerous customised diversity of variants provide a maximum degree of flexibility in comparison with standard systems.
The basic unit consists of a glass vessel with a stainless steel cover with all the required connections and an exhaust air cooler. Containers with working volumes from 150 to 1,600 ml are available.
The pH value is measured via an invasive pH electrode and can be regulated by CO2 /base or acid/base.
Gas injection can be applied by the headspace, a sparger or by a gas injection ring.
A solid-state temperature control system without heat transfer medium is used for heating and cooling, i. e. the vessel is heated and cooled over the base plate.
Highly precise pump drives for analysing pump hoses with dosing rates of 1 μl/min up to 30 ml/min enable exact dosing in semicontinuous and continuous operation. Laboratory balances may be optionally integrated in order to achieve controlled gravimetric dosing.
The stirrer is powered by a speed-controlled, wear-free, brushless DC motor A mechanical seal ensures the required sterility. Propeller, Rushton or diagonal blade stirrers are used. A magnetic stirrer with PTFE stirring rods is available as an alternative or supplement for gravity-sensitive micro-organisms.
OmniFerm® mini fermenters are sterilised by autoclaving. After disconnecting the plug and the stirrer drive and the removal of the hoses from the pump drives the vessels may be sterilised within the autoclave.
Advantages
- Full research fermenter functions
- High level of automation
- Extensive sensor system possible
- Temperature control without heat transfer medium
Gas Mixing Systems
Graduated solutions are available for gas mixing. For most applications the triple multiplexed gas mixture OMNIFERM-GASMF with a common mass flow controller for all gases except for CO2 is sufficient. The compact module is fitted directly onto the rear panel of the fermenter control unit.
Variable area flowmeters with a manually activated needle valve can be fitted for simple applications.
A design with a separate CO2 mass flow controller is available for cell culture equipment. For sophisticated demands the OmniFerm® mini can be fitted with a mass flow controller for each gas separately.
| Dimensions (D x W x H) | 390 x 275 x 900 mm | ||
|---|---|---|---|
| Reactor volume (ml) | 600 | 1,000 | 2,000 |
| Working capacity (ml) | 275 - 500 | 500 - 800 | 500 - 1,600 |
| Container height outside (flange) | 145 mm, DN80 | 155 mm, DN100 | 260 mm, DN100 |
| Electrode length (mm) | 120 | 120 | 225 |
| Electrical connections | 230 V AC, 3 A | ||
| Materials | Borosilicate glass and stainless steel 1.4571 | ||
| Sterilisation | Autoclave | ||
| Connections | 3 x PG13.5 thread, 6 x 4 mm, 1 x 6 mm (supply air), 1 x 12 mm (exhaust air cooler). Optionally deviating | ||
| Sensors | Temperature, optional: pH, dissolved O 2 , dissolved CO 2 , Redox, fill level, foam, OD | ||
| Pumps | 4 peristaltic hose pumps 0...30 ml/min, option with gravimetric control | ||
| Stirrers | Wear-free BLCD drive with Rushton turbine or stirrer as required | ||
| Temperature/(refrigeratingcapacity) | RT -10 °C to 50 °C (refrigerating capacity 20 W), optional cooling with water RT-20 °C to 50 °C (refrigerating capacity 40 W), optional Cold finger PG13.5 thread (refrigerating capacity 100 W) | ||
| Gas injection | Immersion pipe. optional sparger, gas injection ring or headspace gas flushing | ||
| Shaft sealing | Mechanical seal | ||
| Product code | Description |
|---|---|
| CG-OMNIFERM-ABK | Operator station (OS) for up to 4 OmniFerm®‐units, without software |
| SL-LABVIPOMNI | LabVision® OmniFerm® package, is required once for each OS |
| SL-RUNTIME | 50 % reduced runtime-version of the LabVision® OmniFerm® package.* |
| SL-LABVIPOMNI-E | Extension of the LabVision® OmniFerm® package for one further unit for each OS |
| VA-OMNIFERM-FE-600 | OmniFerm® mini fermentation unit, working capacity 275 to 500 ml, with 4 dosing pumps |
| VA-OMNIFERM-FE-1000 | OmniFerm® mini fermentation unit, working capacity 500 to 800 ml, with 4 dosing pumps |
| VA-OMNIFERM-FE-2000 | OmniFerm® mini fermentation unit, working capacity 500 to 1,600 ml, with 4 dosing pumps |
| VA-OMNIFERM-MAG | Magnetic stirrer drive for magnetic stirring rods |
| VA-OMNIFERM-RUSH | Rushton stirrer |
| VA-OMNIFERM-PROP | Propeller stirrer |
| VA-OMNIFERM-BUFF | Baffle** |
| VA-OMNIFERM-6RS232 | 6 RS-232 connections for laboratory balances etc. |
| IL-OXYTECT-xx | Dissolved oxygen sensor with evaluation electronic, autoclavable, PG13.5 thread |
| IL-CELLTECT-xx-ww | Sensor for optical density with evaluation electronics, 4...20 mA,, autoclavable, PG13.5 thread |
| IL-PHFERM-l | pH sensor, pH value 0...14, 0 up to 130 °C, 0...10 bar, autoclavable, PG13.5 thread |
| VA-OMNIFERM-BGR | Stainless steel gas injection ring |
| VA-OMNIFERM-SIL | Silicone tube gas injection ring |
| VA-OMNIFERM-SPARG | Gas sparger |
| VA-OMNIFERM-AIRPUM | Electronically controlled gas injection pump for up to 4 fermenters, max 550 l/h |
| VA-OMNIFERM-GAS1 | Gas injection control with float-type flow meter and pulsed valve |
| VA-OMNIFERM-GASMF | Gas injection control with mass flow controller, 1 gas |
| VA-OMNIFERM-GASMF2 | Gas injection unit with 2 mass flow controllers, e. g. air and CO 2 |
| VA-OMNIFERM-GASMF4MUX | Gas injection unit with mass flow controller, quadruple multiplex, 4 gases |
| VA-OMNIFERM-GASMFZK | Gas injection unit 4 x for cell culture, multiplex with additional CO 2 mass flow controller |
| VA-OMNIFERM-REDOX | Redox probe, autoclavable, PG13.5 thread |
| VA-OMNIFERM-LEV | Level probe, autoclavable |
| VA-OMNIFERM-FOAM | Foam probe, autoclavable |
| VA-OMNIFERM-FOLE | Combined probe for level and foam, autoclavable |
| IL-HISENSE-c-x | Exhaust gas analysis O 2 , OTR and CO 2 , CTR, RQ for cell culture |
| IL-HISENSE-m-x | Exhaust gas analysis O 2 , OTR and CO 2 , CTR, RQ for microbiology |
xx; see OxyTect™ probe
xx, ww: see CellTect™ probe
* The Runtime-version permits operations as usual but no further devices can be connected. Connected devices cannot be replaced by different types.
An individual parameterisation of the devices is not possible.
** not available for VA-OMNIFERM-FE-600
A system comprising two 2-litre fermentation units which are connected to an OS. Additionally, it is intended that a stainless steel gas injection ring should be used in place of the standard silicone ring. Gas injection should be able to be regulated with the aid of a mass flow controller as a variation from the standard rotameter. The system is intended, for example, to be used for training and therefore the software bundle as runtime is sufficient.
| Quantity | Product code | Description |
|---|---|---|
| 2 | VA-OMNIFERM-FE-2000 | OmniFerm® mini fermentation unit, working capacity 500 to 1,600 ml, with 4 dosing pumps |
| 2 | VA-OMNIFERM-BGR | Stainless steel gas injection ring |
| 2 | VA-OMNIFERM-GASMF | Gas injection control with mass flow controller, 1 gas |
| 2 | IL-OXYTECT-220 | Dissolved oxygen sensor with evaluation electronic, autoclavable, PG13.5 thread |
| 2 | IL-PHFERM-220 | pH sensor, pH value 0...14, 0 up to 130 °C, 0...10 bar, autoclavable, PG13.5 thread |
| 4 | ZK-X-HI62-IA-KA-I-01 | Omniferm® VP8-cabel for pH and oxygen sensor |
| 2 | VA-OMNIFERM-FOAM | Foam probe, autoclavable |
| 1 | CG-OMNIFERM-ABK | Operator station (OS) for up to 4 OmniFerm® units, without software |
| 1 | CP-M23TFTWS | 23 inches wide-screen TFT colour monitor |
| 1 | SL-LABVIPOMNI | LabVision® package, is required once for each OS |
| 1 | SL-RUNTIME (optional) | 50 % reduced runtime version of the LabVision® OmniFerm® package.* |
| 1 | SL-LABVIPPOMNI-E | Extension of the LabVision®‐OmniFerm®-Package for a further OmniFerm® unit for each OS |
* The runtime version permits operations as usual but no further devices can be connected. Connected devices cannot be replaced by different types.
An individual parameterisation of the devices is not possible.